Dr. Mark Steinberg
Dr. Steinberg’s research is in the area of cell and molecular biology with a focus on the mechanisms by which human cells become transformed to the malignant state. He is an Area Group I and Executive Committee member.
The goal of our research is to understand the process by which human epithelial cells come to acquire the properties of tumor cells derived from malignant neoplasms. For this purpose this laboratory has utilized cultured normal and immortalized human epidermal keratinocytes to study the process of oncogenic transformation in model systems in vitro.
I. Activation of oncogenic pathways related to mitochondrial DNA deletions. This project has two main goals:
i. To study patterns of changes in the mtDNA over time following UV irradiation and to correlate alterations in mtDNA that emerge over time with changes in patterns of gene expression of components of growth regulatory signaling pathways potentially related to oncogenic processes.
ii. To test directly the idea that mutations that result in loss of function of a component of the electron transport chain can lead to accumulation of reactive oxygen species (ROS) and are associated with changes in gene expression related to altered growth control.
II. Oncogenic gene expression in human keratinocytes carrying a unique SV40 integrant The goal of this project is to study the relationship between host gene expression regulated by the SV40 T antigen in human epithelial cells bearing a unique viral integrant. Towards this end we are employing qPCR expression arrays to study modulation of gene expression following siRNA-mediated knockdown of the viral early genes.
Dr. Mark Steinberg
Department of Chemistry, Room MR-1024
City College of New York
138th Street & Convent Avenue
New York, NY 10031
Other Locations: MR-629