Dr. Paul Gottlieb
Dr. Gottlieb’s group has established a research program that will yield insights into the Cystoviridae, influenza, and viral relationships to Systemic Lupus Erythematosus (SLE).
Dr. Gottlieb’s research group is studying several Cystoviridae species utilizing methods of cryo-electron microscopy. (This project is being performed in collaboration with Dr. Alvin Katz of the Department of physics and Dr. Sacha De Carlo of the Department of Chemistry.) The viruses contain a genome consisting of three segments of double-stranded RNA (dsRNA). Their mechanism of RNA packaging, replication and transcription is similar to that of the Reoviridae of which one member, rotavirus, is a major cause of severe infant gastroenteritis. The microscopy studies will determine the ultrastructure of the RNA synthesis portal that is responsible for packaging and replicating the viral genome. Understanding the RNA synthesis machinery in detail will be crucial in the development of new antiviral compounds.
In a collaborative study with Dr. Spatz, the research group is investigating the role of the Epstein Barr Virus (EBV) in the etiology of Systemic Lupus Erythematosus (SLE). This research project focuses on characterizing the anti-double stranded DNA (dsDNA) response in mice that express the viral Epstein Barr Nuclear Antigen-1 (EBNA-1). The expression of this antigen in animals has been achieved through the use of DNA-based vaccination of recombinant plasmids and proteins. The establishment of this in vivo model enabled us to demonstrate that the inoculated mice produced antibodies to autoantigens associated with SLE.
Dr. Gottlieb has recently established a study of influenza vaccine viral strains in collaboration with Dr. Doris Bucher of the New York Medical College. Dr. Bucher produces influenza vaccine strains including a strain that was utilized in the recent swine flu pandemic. Drs. Gottlieb, Katz, and De Carlo are utilizing cryo-EM techniques to analyze the assembly of influenza reassortant strains in order to determine the physical qualities that quarantine high yield antigenicity. This information will permit the rapid and efficient production of the new influenza vaccines that must be produced on a yearly basis.
Dr. Gottlieb’s laboratory also has established an interdisciplinary study including the CCNY Institute of Ultrafast Lasers (IUSL) and Spectroscopy and the CCNY Department of Earth Atmospheric and Space Sciences (EAS). This collaborative study is examining the effect of environmental clays in the formation of biofilms by microorganisms. The project is directed by the EAS chair Dr Jeffrey Steiner and EAS faculty member Dr. Karin Block. Dr. Katz of the IUSL is performing the spectroscopy component of the project.
Dr. Paul Gottlieb-Assistant Professor
Department of Microbiology
& Immunology – Harris Building 210
City College of New York
138th Street & Convent Avenue
New York, NY 10031